Tag

Part:BBa_K4361104

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-06)


6xHis-tag with TEV protease cleavage site

Composite part consisting of Part:BBa_K4361102, a linker sequence, and Part:BBa_K4361101.With the combination of the His-Tag and the TEV site a protein can first be purified with His-Tag purification technique and second be digested with TEV protease to cut off the His-TEV site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None